qPCR Probe Design Service

Enter your sequence in FASTA format
Choose database to check design against
Default: No Database
qPCR target region
Default: The whole sequence
startposition endposition
qPCR primer
minimum maximum
length
GC content
melting temperature Tm
qPCR product
minimum maximum
length
GC content
melting temperature Tm
qPCR probe
minimum maximum
length
GC content
Specify orientation of probe