PCR Primer Design Service
Enter your sequence in
FASTA
format
Choose database to check design against
Default:
No Database
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RefSeq [H. sapiens]
RefSeq [M. musculus]
RefSeq [R. norvegicus]
PCR target region
Default:
The whole sequence
startposition
endposition
PCR primer
minimum
maximum
length
GC content
melting temperature Tm
PCR product
minimum
maximum
length
GC content
melting temperature Tm